Aggregation Temperature (Tagg) – what is it, and how can we calculate it?

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Measuring aggregation temperature using the Zetasizer

Tagg-Tm

In dynamic light scattering (DLS), the hydrodynamic size and size distribution of particles in solution can be obtained.  It may be of interest to examine this measurement as a function of time and temperature, or as a ‘trend’, as it is called in the Malvern Zetasizer software.  For biological protein samples, this temperature trend is particularly interesting: although at low temperatures a protein may be stable and show repeatable size (and scattering intensity) measurements, typically at some elevated temperature (Tagg), protein molecules will show a tendency to oligomerize or aggregate. The temperature at which this occurs will depend on the protein itself, plus the buffer composition, and it is usually only observed clearly when the starting protein is homogeneous at the beginning of the measurement. An example, discussing the stability of recombinant human albumin, is discussed in the application note, “Using light scattering to study the thermal stability of Recombumin – predicting shelf life“.

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Malvern Panalytical embraces the Internet of Things with TetraScience partnership for smart laboratories

 

Malvern Panalytical, a global leader in analytical characterization technology development, has announced a collaboration with specialist Internet of Things (IoT) company, TetraScience, to deploy IoT-enabled capabilities and connectivity for its instruments. The objective of the collaboration will be to provide access to customized dashboards, data collection and analytics, and system scheduling and monitoring.  This will allow researchers – particularly those working in the pharmaceutical industry and other sectors involved in the creation of complex materials – to improve laboratory efficiency, better manage their equipment, and make swift, data-driven decisions.

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Guide to molecular weight – with the Zetasizer Nano

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Molecular weight measurements with the Zetasizer software?

First a few words of caution: molecular weight or Mw measurements in general are not easy, require time, good sample preparation skills as well as patience. If you are new to the Malvern Zetasizer it might be best to familiarize yourself first with the rapid, user-friendly, and quite forgiving technique of dynamic light scattering (DLS).

OK, with that out of the way, the software can handle static light scattering with the relatively simple concentration series method. All that’s required is

  • a scattering standard (which is usually defaulted to Toluene)
  • the dispersant, or solvent (where the sample material is dispersed in)
  • a series of concentrations in the dispersant (5 or more different ones would be a good number)

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Yearend specials from Micron Scientific

At this time of the year a small number of demonstration instruments becomes avialable for resale at a considerable discount. Contact us if you have an interest in a demo  laser diffraction Mastersizer 3000, Kinexus Rheometer, Zetasizer Nano ZS or NTA NS 300. Please note this is a limited time offer. 

kinexus range zetasizernanozsnanosight

After sales support for Pamas's range of particle counters

After sales support is an important consideration before a new client purchase any analytical instrumentaton. Micron Scientific has invested in after sales support over the years to offer our  Pamas clients seemless support.

Besides the yearly calibration of your particle counter our Pamas factory trained service engineers can also diagnose and repair any technical faults that might occur.

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NanoSight unique contributions to today’s hottest research fields

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Being involved with the NanoSight products gives us visibility over a wide range of research areas that have a direct benefit to science and humanity. It is quite exciting to feel we are contributing to advancing science in these fields.

I was prompted to do a quick review of recent publications to see where NanoSight systems are being used after reading a recent article by Lara Aqrawi on characterizing extracellular vesicles in tears and saliva as a non-invasive diagnostic method for primary Sjögren’s syndrome (pSS) patients. Nanoparticle tracking analysis was conducted to determine size distribution and concentration of EVs[1].

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New users of DLS & the Zetasizer? Quick guide to perfection!

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Sticky Note

Whether in a university or a quality assurance laboratory a new instrument user will tend to ask the same questions:

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The Mysterious Case of the Musca Domestica Contaminant

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Just when I thought I’d seen everything that could contaminate the background, a user sent me this picture! The Musca Domestica (common housefly) was attracted by certain aromatics used in testing and seems to have entered the measurement cell through an empty Hydro EV over a weekend. Unfortunately, it became wedged within the measurement cell and did not survive the experience.

Malvern-lens-with-fly-580 

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Parenterals: What are lipid emulsions?

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Intralipid-Lipid emulsions

iStock_73218599_580x200 

Lipid emulsions are administered intravenously for a variety of purposes:

  • To help feed patients with temporary difficulty in ingesting food orally
  • For better transport of lipophilic drugs (fat-friendly active ingredient (API) which is not soluble in water)
  • For controlled release of drugs (size related to uptake and retention)
  • To decreasing adverse reactions to drugs
  • To improve targeting of drugs
  • In emerging gene therapies for cellular and gene delivery, and personalized medicine

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Viscoelasticity and the pitch drop experiment

Written by: Shona Murphy

Last year I went on a rheology tour!! I gave 12 talks in 10 days in 7 locations around Australia and New Zealand, talking primarily about suspension rheology and how particle characteristics such as particle size and shape affect rheology.

I spoke about the stability of suspensions and the factor to consider when assessing suspension stability but another major focus was the viscoelastic properties of materials.

Viscoelasticity: A material that possesses both liquid and solid-like properties which may change depending on how we handle and treat it.

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Customer Spotlight: Collaborating for safer biopharmaceuticals

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Our key driver at Malvern Instruments is to create technologies that make a difference or solve a problem, and working hand-in-hand
with our customers enables us to make sure that’s exactly what’s happening, out in the field.  We place a high value on our collaborations
and partnerships with customers from a wide range of industries and organizations across the globe and love to see how our systems
are being used in real-world scenarios.  This gives us vital feedback on what we’re doing well, and also any tweaks we need to make in
order to optimize our technologies.

 

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The inaugural Malvern TEDx evening!

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On April 26th, 2017, I had the great pleasure of being invited to talk as part of the very first Malvern TEDxevening, held at the Coachhouse Theatre in Great Malvern.  
It was a really enjoyable event, and the talks are now on YouTube for you all to view!

Youtube video

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Kinexus + series Top 5 benefits

 

Viscosity and rheology measurements often historically varied from user to user, and instrument to instrument.

Having this in mind, Malvern’s Kinexus rheometer was designed with five unique features that make it both \

easier to use and help generate more reproducible data than comparable systems.

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Food and Drink Seminar coming to Chicago

Written by: Edgar Chamorro

The food industry is extremely diverse (‘we are what we eat’) and requires the understanding and control of specific characterization attributes to impact final product quality. From measuring particle size to bulk properties, our seminar will help you to improve product consistency and the ability to engineer the products for different markets/tastes with a wide range of complementary technologies and application expertise.

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Cement – composition, structure and fineness – Q&A

by Alon Vaisman

In the presentation, we described where and how various technologies fit into the value chain of cement production from quarry to finished grinding and gave practical examples of value and return on investment for the users.

If you missed the webinar, you can view a recording on our website.

Watch Webinar

Below is a list of answers to the questions asked at the event.

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Malvern’s Top Content Q1 2017

by Severine Micheal

You all know now that at Malvern, we like producing regular content which is dedicated to you, depending if you are a student, researcher or scientist.
I really hope that this new Top Content from the last quarter will help you finding the best content from beginner’s guides as always to start with few of our techniques or advanced education materials. It’s still free to access these and more from our Resource Center. So don’t hesitate to visit our website if you want to see the full selection.

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Malvern Webinar Milestone

Written by: Edgar Chamorro

Malvern was one of the first companies of its kind to recognize the value webinars play in providing customers and prospects with a medium for engaging, educational content that could be watched live or at a later date through the recording

We are very pleased recognize to a major milestone for our webinar program.

Going back 15 years to 2001 in Malvern, England, Alan recalls his 1st webinar being “held together with string and wood”. Being lost to time because the webinars were not recorded “on the old days” he recalls that his first webinar was a standard basic principal webinar and not much else about. “Smaller was beautiful” Alan recalls, having under 25 attendees for most of the webinars back then. When asked if he was looking forward to his 100th webinar, Alan commented he is “looking forward to reaching 200 or 1000” to see what future updates we continue to make to the program.

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What is a virus?

Written by: Ulf Nobbmann

How to measure it with DLS?

What virus concentration is needed for reliable DLS size measurements?

 What is a virus?

In cell biology, a virus is a self assembly of organic molecules (mainly proteins) in the size range 20 nm – 300 nm (diameter). Viruses hold great interest for human gene therapy researchers, and the AAV-2  virus shown to the left is an example.

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Starting a new partnership: Case Western Reserve University


Written by: Mark Pothecary

One of the best parts of a job as a Product Manager of Technical Specialist is the continual learning that comes from customer interactions, relationships and collaborations. Visiting a new lab, learning about new areas of research and helping to guide measurements with real meaning and consequences is both enjoyable and rewarding.

This is particularly true when starting a new academic collaboration.  At Malvern, we’re pleased to announce that we are starting a new collaborative partnership with Professor Rigoberto Advincula at Case Western Reserve University (CWRU) to use advanced multi-detector gel-permeation chromatography (GPC) techniques to study novel polymer conformations.

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Festive webinar - Your letters to Father Characterization

To celebrate the knowledge, support and dedication of our employees Malvern Instruments recently had their first live panel discussion to help you solve your current challenges. 

Whether its related to helping you gain the most value out of your current instrument or to discover more about our latest technologies we encourage you to take this opportunity to ask our experts. We would also love to hear any feedback or success stories that you would like us to read and share with the Malvern community.

Watch now

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Gold Nanoparticle Applications and Characterization by Nanoparticle Tracking Analysis

Gold nanoparticles are one of the most popular and extensively researched type of nanoparticle. A Google Scholar search of the term “gold nanoparticles” results in over 500,000 hits.  Applications range from the biological to the material, and include nanomedicine, drug delivery, cancer diagnostics, biological scaffolds, biosensors, contrast agents, catalysts, and CMP slurries for the manufacturing of microprocessors 

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Surface Measurement Systems launches the new DVS Adventure

DVSAdventureDVS Adventure is a water vapor sorption analyzer measuring sorption and desorption isotherms over a broad range of humidities and temperatures. It offers unprecedented temperature stability and humidity performance. It provides valuable information about the interactions of a sample with water vapor that is critical for the development of new materials and processes.

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Five ways that the Mastersizer 3000 saves you money

mastersizer 3000e ms websiteTo earn its place in today’s busy analytical laboratories, an instrument must meet certain practical criteria, and stand-up to rigorous economic evaluation. For labs that need particle sizing, laser diffraction systems are typically the technology of choice; delivering accurate and reliable information for the broadest range of sample types.

Here, we look at how the Mastersizer 3000, the market leading laboratory particle size analyzer from Malvern Instruments, can save you time, money and resources, and ultimately deliver a healthy return on investment.   

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New Lasair® III Aerosol Particle Counter with Internal WiFi

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Synuclein – A protein that fuses vesicles

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α-synuclein promotes vesicle assembly – when size can support theory

Double-anchor-mechanism-model 

What is a vesicle?

In cell biology, a vesicle is an (often spherical) assembly of amphiphilic molecules (usually proteins and phospholipids), and is typically sized between 30 nm – 100 nm in diameter. Vesicles are often classified by the type of interaction they are involved with. For extracellular vesicles (or exosomes), this is usually intercellular signal transmission. A particular example of exosomes is synaptic vesicles, which transmit signals between neurons (average size: 40 nm).

In a paper entitled “Structural basis of synaptic vesicle assembly promoted by α-synuclein1”  the authors investigate a specific protein which forms fibrillar aggregates and is implicated in Parkinson’s disease. A double-anchor mechanism as illustrated in the figure on the left (taken from the publication under creative commons license from Figure 3 in: Nature Communications 7, Article number 12543, doi:10.1038/ncomms12563) shows the protein pulling together two vesicles before fusion. Modeling has supported the reach of this mechanism at up to 15 nm.

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New demonstration Nanosight NS300 instrument for Micron Scientific

220x138 NanoSight NS300 01 220x138Micron Scientific recently received its own Nanosight NS300 instrument. "We are very excited to add the NS300 to our range of demonstration instruments " said Albrecht Truter, Director of Micron Scientific. Nano Tracking Analysis (NTA) is  a very visual technique as the user is able to observe the light scattering from their nano particles directly in the software.  It can measure samples at low concentrations as it measures individual particles. This particle by particle approach allows for a relatively high resolution of the nano particle size distribution. 

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